肝细胞癌患者癌组织CK7、ARG1及GPC3的表达及临床意义

Expression and clinical significance of CK7, ARG1 and GPC3 in hepatocellular carcinoma

中文关键词: 肝细胞癌  癌组织  CK7  ARG1  GPC3

中文摘要:

      目的 探讨肝细胞癌(HCC)患者癌组织细胞角蛋白7(CK7)、精氨酸酶1(ARG1)、磷脂酰肌醇蛋白聚糖3(GPC3)的表达,并分析其临床意义。方法 回顾性分析本院2020年10月-2023年10月收治的84例HCC患者,均行开腹肝叶切除术治疗。由手术中取出的癌组织与癌旁正常组织获取新鲜标本,检测CK7、ARG1及GPC3表达情况。比较癌组织和癌旁组织中CK7、ARG1及GPC3阳性检出率；根据预后结局分为存活组(n=50例)、死亡组(n=34例)；对比存活组、死亡组的病理特征；对比CK7、ARG1、GPC3阳性及阴性表达的HCC患者1年生存差异。采用多因素Logistic回归模型分析HCC患者预后的影响因素。结果 癌组织中CK7、ARG1及GPC3阳性检出率均高于癌旁组织(P<0.05)。死亡组组织低分化、TNM分期Ⅲ-Ⅳ期、淋巴结转移、被膜浸润占比均高于存活组(P<0.05)。CK7、ARG1及GPC3阳性HCC患者1年生存率均低于对应的阴性患者(P<0.05)。Logistic回归结果显示,组织低分化、TNM分期Ⅲ-Ⅳ期、淋巴结转移、被膜浸润、CK7、ARG1及GPC3阳性均为HCC患者死亡的危险因素(P＜0.05)。结论 CK7、ARG1及GPC3在HCC患者癌组织中均呈阳性表达,且HCC患者预后影响因素与低分化、Ⅲ-Ⅳ期、淋巴结转移、被膜浸润及CK7、ARG1及GPC3阳性密切相关。

英文摘要:

      【 】 Objective To investigate the expression of keratin 7(CK7), arginase 1(ARG1) and phosphatidyl inositol proteoglycan 3(GPC3) in hepatocellular carcinoma (HCC), and to analyze their clinical significance. Methods A retrospective analysis was performed on 84 HCC patients admitted to our hospital from October 2020 to October 2023, all of whom were treated with open hepatic lobectomy. The expression of CK7, ARG1 and GPC3 was detected by obtaining fresh samples from cancer tissues and adjacent normal tissues. The positive rates of CK7, ARG1 and GPC3 in cancer tissues and adjacent tissues were compared. According to the prognosis, they were divided into survival group (n=50 cases) and death group (n=34 cases). The pathological features of survival group and death group were compared. The 1-year survival difference of HCC patients with positive and negative expression of CK7, ARG1 and GPC3 was compared. Multivariate Logistic regression model was used to analyze the prognostic factors of HCC patients. Results The positive rates of CK7, ARG1 and GPC3 in cancer tissues were higher than those in paracancer tissues (P<0.05). The proportion of low tissue differentiation, TNM stage Ⅲ-Ⅳ, lymph node metastasis and membrane infiltration in death group was higher than that in survival group (P<0.05). The 1-year survival rate of CK7, ARG1 and GPC3 positive HCC patients was lower than that of negative HCC patients (P<0.05). Logistic regression showed that low tissue differentiation, TNM stage Ⅲ-Ⅳ, lymph node metastasis, capsular infiltration, positive CK7, ARG1 and GPC3 were all risk factors for death in HCC patients (P < 0.05). Conclusion CK7, ARG1 and GPC3 are all positively expressed in HCC tissues, and the prognostic factors of HCC patients are closely related to low differentiation, stage Ⅲ-Ⅳ, lymph node metastasis, capsule infiltration and positive CK7, ARG1 and GPC3.